Biaffin GmbH & Co KG
About us
Biaffin GmbH & Co KG is an ISO 9001:2015 certified bioanalytical service provider specialized in the application of biosensors based on surface plasmon resonance (SPR) for biomolecular interaction analysis (BIA). BIA technology is ideally suited for highly accurate kinetic characterization of any interacting molecules in real-time in a label-free state. Biaffin has extensive knowledge and many years of experience in various application areas and supports biopharmaceutical drug development programs of pharma, biotech and generic drug companies as well as scientific institutions worldwide as a competent partner. The service is performed by a highly specialized team (PhD level scientists) in a laboratory well equipped with several state-of-the art SPR instruments (5x Biacore T200, 1x Biacore 8K+, 1x Biacore 1S+). Sufficient capacity of SPR instruments allows Biaffin to start interaction studies on short notice, quickly run multiple projects in parallel and receive results in a timely manner. A qualified Biacore T200 instrument is available for SPR assay validation and SPR analysis under GMP conditions. Highly accurate SPR assays for many target proteins and biosimilars are already established and available off the shelf, and established protocols can rapidly be adapted to new target proteins using specific capture assays. The company’s expertise is documented by many peer-reviewed publications in renowned scientific journals.
Address
Heinrich-Plett Str. 40
34132 Kassel
Germany
E-mail: drewianka@biaffin.de
Phone: +49 561 8044668
Internet: www.biaffin.com
Contact person:
Products & Services
SPR Biacore services for biopharmaceutical development
For the development of therapeutic antibodies, biopharmaceuticals and biosimilars, Biaffin offers reliable SPR services for a highly accurate, quantitative kinetic characterization, semi-quantitative kinetic ranking or rapid qualitative screening of drug candidates. SPR assays are already established for many target proteins and can be developed quickly by application of established protocols for reversible and site-directed coupling of biomolecules using specific capture surfaces. These assays can be applied for comparability and stability studies and batch to batch comparison during process optimization. Available assay formats include dual-potency assays, pair-wise epitope mapping and determination of active concentration based on binding activity (calibration-free methods). Assays can be validated and conducted under GMP-like conditions on a qualified Biacore instrument.
ADCC SPR assays
ADCC SPR assays are established for interaction analysis of antibodies with Fc gamma receptors (CD16, CD32, CD64) including receptor variants and different species, neonatal Fc receptor (FcRn) and complement C1q binding.
Small molecule drug development
Biaffin’s services in small molecule drug development comprise customized SPR assay development for defined target proteins, fragment screening, hit validation, secondary screening, kinetic profiling, competition analysis and lead optimization of compounds to improve specificity, selectivity and potency. Early ADME studies (serum protein binding) and studies on the mode of action, cofactor requirements and thermodynamic binding analysis further support the drug development process.
Catalogue products
Apart from analytical services, Biaffin offers a range of products serving customers’ needs in kinase research and exploring cellular signal transduction pathways. Biaffin’s product portfolio, available from the online shop www.Proteinkinase.biz, comprises recombinant proteins (kinases, cytokines, receptors, CD antigens), peptide substrates, specific inhibitors and a biochemical ATP determination assay for customers’ in-house research.