T cells are activated upon contact with other cells that present an appropriate peptide-MHC complex. The immunogenicity of the peptides is thereby restricted by their binding motives to different MHC proteins among individuals. At PEPperPRINT, we set up a peptide library by converting the original protein into overlapping peptides, and analyzing the peptide immunogenicity with a combination of peptide-MHC binding prediction tools and validation assays such as ELISpot.